Helical Structures in the Nucleus of Amoeba proteus

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Helical Structures in the Nucleus of Amoeba proteus

While Amoeba proteus has long been the subject of extensive cytological and cytochemical studies, investigations with the electron microscope have been limited principally to the nuclear membrane (1-3). The observations reported herein were made in the course of studies intended to correlate cytochemistry (4) with knowledge of fine structure obtained with the electron microscope. I t is felt th...

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Nucleus-associated actin in Amoeba proteus.

The presence, spatial distribution and forms of intranuclear and nucleus-associated cytoplasmic actin were studied in Amoeba proteus with immunocytochemical approaches. Labeling with different anti-actin antibodies and staining with TRITC-phalloidin and fluorescent deoxyribonuclease I were used. We showed that actin is abundant within the nucleus as well as in the cytoplasm of A. proteus cells....

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Distribution of proteins between nucleus and cytoplasm of amoeba proteus

By transplanting nuclei between labeled and unlabeled cells, we determined the localization of the major proteins of amebas and described certain features of their intracellular distributon. We identified approximately 130 cellular proteins by fluorography of one-dimensional polyacrylamide electrophoretic gels and found that slightly less than half of them (designated NP, for nuclear proteins) ...

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Distribution of Proteins between Nucleus and Cytoplasm of Amoeba proteus

By transplanting nuclei between labeled and unlabeled cells, we determined the localization of the major proteins of amebas and described certain features of their intracellular distribution . We identified -130 cellular proteins by fluorography of one-dimensional polyacrylamide electrophoretic gels and found that slightly less than half of them (designated NP, for nuclear proteins) are almost ...

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Filamentous Structures in Amoeba Proteus Visualized in Scanning Microscope.

The dynamic cytoskeleton in Amoeba proteus is a filamentous gel network, composed mainly of F-actin microfilaments accompanied by actin binding proteins, distributed in the peripheral cortex beneath the cell membrane and around the nucleus. As well the cell shape changes as the motility primarily depend on the activity of these structures. In contrast, the endoplasm of amoeba is commonly consid...

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ژورنال

عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology

سال: 1956

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.2.2.221